Iq5 real time pcr detection system manual

Bio-Rad iQ™5 Optical System Software, Version User Manual. Loading Bio-Rad’s real-time thermal cyclers are licensed real-time thermal cyclers under Applera’s United States Patent 6,, B1 for use in research, human in vitro diagnostics, and for all other fields except the field of veterinary diagnostics. Handle all components of the real-time PCR detection system with care and with clean, dry hands at all times. The optical system contains mirrors and lenses that may shatter if the unit is dropped or struck with great force. If the unit is damaged such that internal components or wires are exposed, contact your local Bio-Rad office immediately. The iQ5 system offers five-target analysis capabilities for multiplex PCR and data collection and analysis options for gene expression analysis. The iQ5 system is compatible with multiple detection chemistries, providing maximum flexibility in real-time PCR applications. Key Features of the Bio-Rad iQ5 RT-PCR: Multiplexing of up to 5 fluorophores in each reaction vessel/5(4).

Power SYBR® Green PCR Master Mix: Executed on Bio-Rad iQ5 Real-Time PCR Detection System For safety and biohazard guidelines, refer to the "Safety" section in the Power SYBR® Green PCR Master Mix and RT-PCR Protocol (PN ). Read the MSDS and follow the handling instructions. Wear appropriate protective eyewear, clothing, and gloves. The iQ5 real-time PCR detection system is a modular upgrade to the iCycler thermal cycler with well reaction module. With the iQ5 system, you can accurately quantitate targets over a dynamic range of more than 9 orders of magnitude. You can also perform qualitative assays for allelic discrimination or screening for known mutations. The Bio-Rad Icycler Thermal Cycler iQ5 real-time PCR detection system offers a fresh design for Bio-Rad's new generation of real-time PCR instrumentation. Like its predecessors, the iCycler iQ and MyiQ systems, the iQ5 optical system is a modular upgrade to the iCycler thermal cycler with well reaction block.

The iQ5 real-time PCR detection system offers a fresh design for Bio-Rad's new generation of real-time PCR instrumentation. Like its predecessors, the iCycler iQ and MyiQ systems, the iQ5 optical system is a modular upgrade to the iCycler thermal cycler with well reaction block. perform the External Well Factor Plate procedure consult the Bio-Rad iCycler iQ5 Real-Time PCR Detection System Instruction Manual. Two-Step RT-PCR FastStart Universal SYBR Green Master (ROX) can also be used to perform two-step RT-PCR. In two-step RT-PCR, the reverse transcription of RNA into cDNA is separated from the other reaction steps and is performed outside the real-time PCR instrument. Subsequent amplification and online monitoring is performed according to the standard real-time. Plexor® HY System and provides instructions for use with the Bio-Rad iQ™5 Real-Time PCR Detection System, including reaction setup, real-time PCR instrument programming and data analysis using the Plexor® Analysis Software. The Plexor® technology takes advantage of the specific interaction between two modified nucleotides to achieve quantitative PCR analysis (1–3). One of the PCR primers contains a.